Shimadzu D2 lamp HPLC instrument refers to Shimadzu brand HPLC instrument equipped with deuterium (D2) lamp as light source. D2 lamp is a commonly used ultraviolet light source, which can provide a continuous spectrum of 190-400nm and is suitable for ultraviolet-visible absorption detection. The following is the relevant introduction:

Main components and functions:
Pump system: For example, LC-20A series pumps can provide stable mobile phase transportation, and can perform binary or ternary high pressure gradient or quaternary low pressure gradient. Taking LC-40 series pumps as an example, there are different models, such as LC-40B XR, LC-40D X3, and their allowable maximum pressure, flow rate setting range and other parameters are different in SHIMADZU CORPORATION.
Sampler: For example, SIL-20A automatic sampler can realize full sample injection and variable sample injection, and can handle sample bottles with different specifications.
Detector: usually equipped with UV-VIS detectors, such as SPD-20A, SPD-M20A, etc., with D2 lamp as the light source. The wavelength range of SPD-20A is 190-700nm, and the wavelength accuracy is below 1nm. SPD-M30A has 1024 diode elements, the wavelength range is 190-700nm, and the wavelength accuracy is ≤±1nmSHIMADZU.

Working principle: A high-pressure pump is used to pass the mobile phase through a chromatographic column with a stationary phase at a stable flow rate. After the sample is injected into the mobile phase, the components in the chromatographic column are separated due to different forces between the stationary phase and the mobile phase. The separated components flow through the detector in turn, and the ultraviolet light emitted by the D2 lamp is absorbed by the components. The detector detects and quantitatively analyzes the components according to the strength of the absorption signal.
Application: Widely used in chemistry, pharmacy, biology, environment and other fields. In pharmaceutical industry, it can be used for drug quality control, purity detection, drug metabolism research, etc. In environmental monitoring, it can be used to detect pollutants in water, such as polycyclic aromatic hydrocarbons and pesticide residues.
Characteristics and maintenance of D2 lamp: D2 lamp has the characteristics of stable light emission, high intensity and wide spectral range, which can meet the requirements of high performance liquid chromatography ultraviolet detection. Its service life is generally 2000 hours or one year after purchase, whichever comes first. In the process of use, frequent switching of the lamp should be avoided to prolong its service life.